Part:BBa_M36765
Optimized Heat Shock Promoter (2x HSE)
This part is an optimized Heat Shock Expression (HSE) promoter that has been developed as a replacement for HSPA1a, a common promoter used for detection of the transcription factor HSF1. These Heat Shock Proteins (HSPs) are crucial for preventing cell death/enhancing survival. In the study where this sequence was developed, the authors describe a repeating sequence of HSE elements, measuring the change in response with respect to number of elements incorporated. Although 5 HSE's were identified to optimize HSP expression, this sequence uses 2 HSE elements.
These HSE's are more efficient due to selective detection of Heat Shock pathway activity as opposed to their HSPA1a counterparts which have other confounding stress factors included.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 12
Illegal XbaI site found at 31 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 77
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 12
Illegal XbaI site found at 31 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 12
Illegal XbaI site found at 31 - 1000COMPATIBLE WITH RFC[1000]
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